Nutt and his colleagues then calculated the drugs’ overall ratings. Heroin and cocaine had been ranked most dangerous, followed by street and barbiturates methadone. Alcohol was the fifth-most harmful tobacco and drug the ninth most harmful. Cannabis came in 11th, and close to the bottom of the list was Ecstasy. Relating to existing British and U.S. Medication policy, alcohol and tobacco are legal, while Ecstasy and cannabis are both illegal. Earlier reports, including a scholarly research from a parliamentary committee last year, possess questioned the scientific rationale for Britain’s medication classification system. The existing drug program is ill thought-out and arbitrary, said Nutt, referring to the United Kingdom’s practice of assigning drugs to three unique divisions, ostensibly predicated on the drugs’ potential for harm.DNA from 38 of the 74 patients was categorized as hypermethylated.). TFAP2E Hypermethylation and Gene Expression Tumor messenger RNA from 28 of the 74 individuals in the initial cohort was analyzed for TFAP2E expression by means of RT-PCR. Twelve individuals showed TFAP2E hypermethylation and reduced TFAP2E mRNA expression, whereas 3 showed hypomethylation and strong mRNA expression. The remaining 13 patients showed either mRNA hypermethylation and expression or no mRNA expression and hypomethylation. Furthermore, TFAP2E expression was assessed in several patients who had tissue samples from matched primary cancers and metastases.